{"id":31112,"date":"2026-09-08T04:48:00","date_gmt":"2026-09-07T20:48:00","guid":{"rendered":"https:\/\/csccm.org.cn\/?p=31112"},"modified":"2026-09-08T05:48:05","modified_gmt":"2026-09-07T21:48:05","slug":"clin-infect-dis%e5%8f%91%e8%a1%a8%e8%ae%ba%e6%96%87%ef%bc%9a%e8%84%91%e8%84%8a%e6%b6%b2%e7%9a%84%e5%ae%8f%e5%9f%ba%e5%9b%a0%e7%bb%84%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f","status":"publish","type":"post","link":"https:\/\/csccm.org.cn\/?p=31112","title":{"rendered":"[Clin Infect Dis\u53d1\u8868\u8bba\u6587]\uff1a\u8111\u810a\u6db2\u7684\u5b8f\u57fa\u56e0\u7ec4\u4e8c\u4ee3\u6d4b\u5e8f"},"content":{"rendered":"\n

JOURNAL ARTICLE<\/p>\n\n\n\n

Metagenomic Next-generation Sequencing of Cerebrospinal Fluid: First-year Experience at a Tertiary Referral Hospital<\/h1>\n\n\n\n

Sergio Alvarez Mulett, Sofia Molina Garcia, Omar Abu Saleh, et al<\/h3>\n\n\n\n

Clinical Infectious Diseases<\/em>, Volume 82, Issue 5, 15 May 2026, Pages 808\u2013814, https:\/\/doi.org\/10.1093\/cid\/ciaf390<\/a><\/h3>\n\n\n\n

Abstract<\/h2>\n\n\n\n

Background<\/p>\n\n\n\n

Central nervous system (CNS) infections are significant causes of morbidity and mortality, especially when diagnosis is delayed. Traditional cerebrospinal fluid diagnostic methods for CNS infections have limited sensitivity. Metagenomic next-generation sequencing of cerebrospinal fluid (MSCSF) is a newer diagnostic tool capable of detecting a broad range of pathogens in a single assay.<\/p>\n\n\n\n

Methods<\/p>\n\n\n\n

This retrospective cohort study evaluated the first-year experience with MSCSF at Mayo Clinic. A total of 422 tests performed during calendar year 2024 on adult patients at Mayo Clinic facilities and those tested through Mayo Clinic Laboratories were analyzed, with a subcohort analysis of the 210 Mayo Clinic patients performed to define patient characteristics associated with positive results and clinical outcomes.<\/p>\n\n\n\n

Results<\/p>\n\n\n\n

The sensitivity of MSCSF for detecting neuroinfection in the subcohort was 64%, with 43% of confirmed neuroinfections diagnosed by MSCSF only. Subjects who were immunosuppressed (odds ratio [OR]: 3.5,\u00a0P<\/em>\u00a0= .008) and those with a high pretest probability of CNS infection (OR: 20.1,\u00a0P<\/em>\u00a0< .001) were more likely to have positive results. Also, those who tested positive were more likely to die within the ensuing 30 days than those who tested negative (OR: 5.2,\u00a0P<\/em>\u00a0= .001).<\/p>\n\n\n\n

\"\"\/<\/figure>\n\n\n\n
\"\"\/<\/figure>\n\n\n\n

Conclusions<\/p>\n\n\n\n

MSCSF can be a valuable tool for diagnosing CNS infection but should not be used indiscriminately. It appears to be most beneficial when there is a high clinical suspicion of CNS infection and conventional diagnostics, and available molecular tests, yield negative results. Its integration into clinical practice should be guided by diagnostic stewardship to maximize cost-effectiveness and clinical utility.<\/p>\n","protected":false},"excerpt":{"rendered":"

JOURNAL ARTICLE Metagenomic Next-generation Sequencing […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[32,23],"tags":[],"_links":{"self":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/31112"}],"collection":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31112"}],"version-history":[{"count":2,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/31112\/revisions"}],"predecessor-version":[{"id":31651,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/31112\/revisions\/31651"}],"wp:attachment":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31112"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31112"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}