{"id":26621,"date":"2024-08-26T04:30:00","date_gmt":"2024-08-25T20:30:00","guid":{"rendered":"http:\/\/csccm.org.cn\/?p=26621"},"modified":"2024-08-26T05:51:56","modified_gmt":"2024-08-25T21:51:56","slug":"nature%e5%8f%91%e8%a1%a8%e8%ae%ba%e6%96%87%ef%bc%9a%e6%97%a0%e9%9c%80%e8%a1%80%e5%9f%b9%e5%85%bb%e7%9a%84%e8%b6%85%e5%bf%ab%e9%80%9f%e6%8a%97%e7%94%9f%e7%b4%a0%e8%8d%af%e6%95%8f%e6%a3%80%e6%b5%8b","status":"publish","type":"post","link":"https:\/\/csccm.org.cn\/?p=26621","title":{"rendered":"[Nature\u53d1\u8868\u8bba\u6587]\uff1a\u65e0\u9700\u8840\u57f9\u517b\u7684\u8d85\u5feb\u901f\u6297\u751f\u7d20\u836f\u654f\u68c0\u6d4b"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\">Blood culture-free ultra-rapid antimicrobial susceptibility testing.<\/h1>\n\n\n\n<h3 class=\"wp-block-heading\">Kim, T.H., Kang, J., Jang, H.\u00a0<em>et al.<\/em><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><em>Nature<\/em>\u00a0(2024). https:\/\/doi.org\/10.1038\/s41586-024-07725-1<\/h3>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"Abs1\">Abstract<\/h2>\n\n\n\n<p>Treatment assessment and patient outcome for sepsis depend predominantly on the timely administration of appropriate antibiotics<sup><a href=\"https:\/\/www.nature.com\/articles\/s41586-024-07725-1#ref-CR1\">1<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/s41586-024-07725-1#ref-CR2\">2<\/a>,<a href=\"https:\/\/www.nature.com\/articles\/s41586-024-07725-1#ref-CR3\">3<\/a><\/sup>. However, the clinical protocols used to stratify and select patient-specific optimal therapy are extremely slow<sup><a href=\"https:\/\/www.nature.com\/articles\/s41586-024-07725-1#ref-CR4\">4<\/a><\/sup>. In particular, the major hurdle in performing rapid antimicrobial susceptibility testing (AST) remains in the lengthy blood culture procedure, which has long been considered unavoidable due to the limited number of pathogens present in the patient\u2019s blood. Here we describe an ultra-rapid AST method that bypasses the need for traditional blood culture, thereby demonstrating potential to reduce the turnaround time of reporting drug susceptibility profiles by more than 40\u201360\u2009h compared with hospital AST workflows. Introducing a synthetic beta-2-glycoprotein\u00a0I peptide, a broad range of microbial pathogens are selectively recovered from whole blood, subjected to species identification or instantly proliferated and phenotypically evaluated for various drug conditions using a low-inoculum AST chip. The platform was clinically evaluated by the enrolment of 190\u2009hospitalized patients suspected of having infection, achieving 100% match in species identification. Among the eight positive cases, six clinical isolates were retrospectively tested for AST showing an overall categorical agreement of 94.90% with an average theoretical turnaround time of 13\u2009\u00b1\u20092.53\u2009h starting from initial blood processing.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/media.springernature.com\/full\/springer-static\/image\/art%3A10.1038%2Fs41586-024-07725-1\/MediaObjects\/41586_2024_7725_Fig1_HTML.png?as=webp\" alt=\"\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/media.springernature.com\/full\/springer-static\/image\/art%3A10.1038%2Fs41586-024-07725-1\/MediaObjects\/41586_2024_7725_Fig2_HTML.png?as=webp\" alt=\"\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/media.springernature.com\/full\/springer-static\/image\/art%3A10.1038%2Fs41586-024-07725-1\/MediaObjects\/41586_2024_7725_Fig3_HTML.png?as=webp\" alt=\"\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/media.springernature.com\/full\/springer-static\/image\/art%3A10.1038%2Fs41586-024-07725-1\/MediaObjects\/41586_2024_7725_Fig4_HTML.png?as=webp\" alt=\"\"\/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/media.springernature.com\/full\/springer-static\/image\/art%3A10.1038%2Fs41586-024-07725-1\/MediaObjects\/41586_2024_7725_Fig5_HTML.png?as=webp\" alt=\"\"\/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Blood culture-free ultra-rapid antimicrobial susceptibi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[32,23],"tags":[],"_links":{"self":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26621"}],"collection":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26621"}],"version-history":[{"count":1,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26621\/revisions"}],"predecessor-version":[{"id":26624,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26621\/revisions\/26624"}],"wp:attachment":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26621"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26621"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26621"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}