{"id":26248,"date":"2024-10-04T04:31:00","date_gmt":"2024-10-03T20:31:00","guid":{"rendered":"http:\/\/csccm.org.cn\/?p=26248"},"modified":"2024-10-04T06:04:39","modified_gmt":"2024-10-03T22:04:39","slug":"icu-management-practice-%e6%96%b0%e5%9e%8b%e8%84%82%e5%a4%9a%e8%82%bd%e7%b1%bb%e6%8a%97%e7%94%9f%e7%b4%a0%e5%af%b9%e9%87%91%e9%bb%84%e8%89%b2%e8%91%a1%e8%90%84%e7%90%83%e8%8f%8c%e6%9c%89%e6%95%88","status":"publish","type":"post","link":"https:\/\/csccm.org.cn\/?p=26248","title":{"rendered":"[ICU Management &#038; Practice]: \u65b0\u578b\u8102\u591a\u80bd\u7c7b\u6297\u751f\u7d20\u5bf9\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u6709\u6548"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\">Novel Lipopeptide Effective Against Staphylococcus Aureus<\/h1>\n\n\n\n<ul>\n<li>In&nbsp;<a href=\"https:\/\/healthmanagement.org\/c\/icu\">ICU<\/a><\/li>\n\n\n\n<li>Fri, 7 Jun 2024<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/res.cloudinary.com\/healthmanagement-org\/image\/upload\/c_thumb,f_auto,fl_lossy,h_700,q_90,w_1000\/v1717768875\/cw\/00127426_cw_image_wi_742b21b868b09762fd9f1c0bb7d92612.webp\" alt=\"\"\/><\/figure>\n\n\n\n<p>A novel antibacterial lipopeptide produced by the bacterium&nbsp;<em>Serratia marcescens<\/em>&nbsp;is highly effective in killing&nbsp;<em>Staphylococcus aureus<\/em>, one of the most significant pathogens in humans.<\/p>\n\n\n\n<p><em>Staphylococcus aureus&nbsp;<\/em>is among the five most common causes of hospital-acquired infections, often leading to life-threatening infections following surgery. Since the introduction of antibiotics in the early 1940s,&nbsp;<em>S. aureus<\/em>&nbsp;has developed resistance to most classes of antibiotics, including penicillin. Over the last six decades, only two new classes of antibiotics have been introduced. One of these, daptomycin, is also a lipopeptide.<\/p>\n\n\n\n<p>A paper recently published in Microbiology Spectrum provides the first insight into the mode of action of the lipopeptide serrawettin W2-FL10, derived from&nbsp;<em>Serratia marcescens<\/em>. This lipopeptide targets the cell membrane of&nbsp;<em>S. aureus<\/em>, causing lesions that result in the leakage of intracellular components and, ultimately, cell death.<\/p>\n\n\n\n<p>Serrawettin W2-FL10 is not toxic to mammalian cells. This makes it a promising therapeutic agent for treating bacterial infections in humans. Additionally, the smaller structure of this lipopeptide (five amino acids and a C10 fatty acid chain) compared to daptomycin (13 amino acids and a C10 fatty acid chain) suggests significantly lower manufacturing costs for serrawettin W2-FL10.<\/p>\n\n\n\n<p>The study investigated the antibacterial activity, cytotoxicity, and mechanism of action of the non-ionic, cyclic lipopeptide serrawettin W2-FL10 against&nbsp;<em>S.&nbsp;aureus<\/em>.<\/p>\n\n\n\n<p>Isolated bacterial strains from wastewater samples produced biosurfactants effective against antibiotic-resistant and disease-causing bacteria. In polluted environments, bacteria naturally produce biosurfactants to protect themselves and outcompete other bacteria. Pigmented and non-pigmented&nbsp;<em>S. marcescens<\/em>&nbsp;strains produce a wide range of broad-spectrum antimicrobial compounds.<\/p>\n\n\n\n<p>W2-FL10 demonstrated potent activity against Gram-positive bacteria, including&nbsp;<em>S. aureus<\/em>,&nbsp;<em>Enterococcus faecalis<\/em>,&nbsp;<em>Enterococcus faecium<\/em>,&nbsp;<em>Listeria monocytogenes<\/em>, and&nbsp;<em>Bacillus subtilis<\/em>, with minimum inhibitory concentration (MIC) values ranging from 6.3 to 31.3 \u03bcg\/mL. However, it showed no activity against Gram-negative bacteria.<\/p>\n\n\n\n<p>Key parameters contributing to the antimicrobial activity of lipopeptides include hydrophobicity and net charge. Serrawettin W2-FL10 is a relatively small and moderately amphipathic lipopeptide with a neutral charge. This lipopeptide showed potent activity against eight of the nine Gram-positive bacteria tested, with&nbsp;<em>B. subtilis&nbsp;<\/em>showing some resistance. This resistance is possibly due to the production of a surfactin complex containing anionic lipopeptides, which has been shown to antagonise activity by binding to the potent antimicrobial peptide gramicidin S. W2-FL10 exhibited nearly identical activity against five of the nine bacteria in the test panel, indicating similar bacterial targets, concentrations, and modes of action. Conversely, W2-FL10 exhibited its highest activity, outperforming melittin, against the&nbsp;<em>L. monocytogenes<\/em>&nbsp;and&nbsp;<em>E. faecalis<\/em>&nbsp;reference strains.<\/p>\n\n\n\n<p>From these findings, the lipopeptide serrawettin W2-FL10 emerges as a promising candidate for further investigation into its antimicrobial properties.<\/p>\n\n\n\n<p>Source: Stellenbosch University<\/p>\n\n\n\n<p>Image Credit: iStock<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">References:<\/h4>\n\n\n\n<p>Decker T, Rautenbach M, Khan S, Khan W (2024)&nbsp;<a href=\"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02952-23\" target=\"_blank\" rel=\"noreferrer noopener\">Antibacterial efficacy and membrane mechanism of action of the&nbsp;Serratia-derived non-ionic lipopeptide, serrawettin W2-FL10.<\/a>&nbsp;Microbiol Spectr. e0295223.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Novel Lipopeptide Effective Against Staphylococcus Aure [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[24,23],"tags":[],"_links":{"self":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26248"}],"collection":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26248"}],"version-history":[{"count":1,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26248\/revisions"}],"predecessor-version":[{"id":26249,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/26248\/revisions\/26249"}],"wp:attachment":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26248"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26248"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26248"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}