{"id":22304,"date":"2022-08-31T05:09:00","date_gmt":"2022-08-30T21:09:00","guid":{"rendered":"http:\/\/csccm.org.cn\/?p=22304"},"modified":"2022-08-31T05:47:59","modified_gmt":"2022-08-30T21:47:59","slug":"lancet-microbe%e5%8f%91%e8%a1%a8%e8%ae%ba%e6%96%87%ef%bc%9a%e4%ba%a7esbl%e5%a4%a7%e8%82%a0%e6%9d%86%e8%8f%8c%e8%82%a0%e9%81%93%e5%ae%9a%e6%a4%8d%e5%8f%8a%e5%85%b6%e4%b8%8e%e8%82%a0%e9%81%93%e5%be%ae","status":"publish","type":"post","link":"https:\/\/csccm.org.cn\/?p=22304","title":{"rendered":"[Lancet Microbe\u53d1\u8868\u8bba\u6587]\uff1a\u4ea7ESBL\u5927\u80a0\u6746\u83cc\u80a0\u9053\u5b9a\u690d\u53ca\u5176\u4e0e\u80a0\u9053\u5fae\u751f\u7269\u7ec4\u548c\u4ee3\u8c22\u7269\u7ec4\u7684\u76f8\u5173\u6027"},"content":{"rendered":"\n

ARTICLES| VOLUME 3, ISSUE 6<\/a>, E443-E451, JUNE 01, 2022<\/p>\n\n\n\n

Gut colonisation by extended-spectrum \u03b2-lactamase-producing\u00a0Escherichia coli<\/em>\u00a0and its association with the gut microbiome and metabolome in Dutch adults: a matched case-control study<\/h1>\n\n\n\n

Quinten R Ducarmon, Romy D Zwittink, Roel P J Willems, et al.<\/h3>\n\n\n\n

Lancet Microbe 2022; 3: E443-E451 Published:April 20, 2022<\/h3>\n\n\n\n

DOI:https:\/\/doi.org\/10.1016\/S2666-5247(22)00037-4<\/a><\/h3>\n\n\n\n

Summary<\/h2>\n\n\n\n

Background<\/h3>\n\n\n\n

Gut colonisation by extended-spectrum \u03b2-lactamase (ESBL)-producing Escherichia coli<\/em> is a risk factor for developing overt infection. The gut microbiome can provide colonisation resistance against enteropathogens, but it remains unclear whether it confers resistance against ESBL-producing E coli<\/em>. We aimed to identify a potential role of the microbiome in controlling colonisation by this antibiotic-resistant bacterium.<\/p>\n\n\n\n

Methods<\/h3>\n\n\n\n

For this matched case-control study, we used faeces from 2751 individuals in a Dutch cross-sectional population study (PIENTER-3) to culture ESBL-producing bacteria. Of these, we selected 49 samples that were positive for an ESBL-producing E coli<\/em> (ESBL-positive) and negative for several variables known to affect microbiome composition. These samples were matched 1:1 to ESBL-negative samples on the basis of individuals' age, sex, having been abroad or not in the past 6 months, and ethnicity. Shotgun metagenomic sequencing was done and taxonomic species composition and functional annotations (ie, microbial metabolism and carbohydrate-active enzymes) were determined. Targeted quantitative metabolic profiling (proton nuclear magnetic resonance spectroscopy) was done to investigate metabolomic profiles and combinations of univariate (t<\/em> test and Wilcoxon test), multivariate (principal coordinates analysis, permutational multivariate analysis of variance, and partial least-squares discriminant analysis) and machine-learning approaches (least absolute shrinkage and selection operator and random forests) were used to analyse all the molecular data.<\/p>\n\n\n\n

Findings<\/h3>\n\n\n\n

No differences in diversity parameters or in relative abundance were observed between ESBL-positive and ESBL-negative groups based on bacterial species-level composition. Machine-learning approaches using microbiota composition did not accurately predict ESBL status (area under the receiver operating characteristic curve [AUROC]=0\u00b741) when using either microbiota composition or any of the functional profiles. The metabolome also did not differ between ESBL groups, as assessed by various methods including random forest (AUROC=0\u00b761).<\/p>\n\n\n\n

\"\"\/<\/figure>\n\n\n\n
\"\"\/<\/figure>\n\n\n\n
\"\"\/<\/figure>\n\n\n\n
\"\"\/<\/figure>\n\n\n\n

Interpretation<\/h3>\n\n\n\n

By combining multiomics and machine-learning approaches, we conclude that asymptomatic gut carriage of ESBL-producing E coli<\/em> is not associated with an altered microbiome composition or function. This finding might suggest that microbiome-mediated colonisation resistance against ESBL-producing E coli<\/em> is not as relevant as it is against other enteropathogens and antibiotic-resistant bacteria.<\/p>\n\n\n\n

Funding<\/h3>\n\n\n\n

None.<\/p>\n","protected":false},"excerpt":{"rendered":"

ARTICLES| VOLUME 3, ISSUE 6, E443-E451,  […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[32,23],"tags":[],"_links":{"self":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22304"}],"collection":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22304"}],"version-history":[{"count":2,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22304\/revisions"}],"predecessor-version":[{"id":22556,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22304\/revisions\/22556"}],"wp:attachment":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22304"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22304"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22304"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}