{"id":22190,"date":"2022-08-22T04:51:00","date_gmt":"2022-08-21T20:51:00","guid":{"rendered":"http:\/\/csccm.org.cn\/?p=22190"},"modified":"2022-08-22T06:51:45","modified_gmt":"2022-08-21T22:51:45","slug":"jama-intern-med%e5%8f%91%e8%a1%a8%e8%ae%ba%e6%96%87%ef%bc%9a%e6%96%b0%e5%86%a0%e7%97%85%e6%af%92%e6%84%9f%e6%9f%93%e6%9c%9f%e9%97%b4%e5%ae%b6%e7%94%a8%e6%8a%97%e5%8e%9f%e6%a3%80%e6%b5%8b%e4%b8%8ert","status":"publish","type":"post","link":"https:\/\/csccm.org.cn\/?p=22190","title":{"rendered":"[JAMA Intern Med\u53d1\u8868\u8bba\u6587]\uff1a\u65b0\u51a0\u75c5\u6bd2\u611f\u67d3\u671f\u95f4\u5bb6\u7528\u6297\u539f\u68c0\u6d4b\u4e0eRT-PCR\u548c\u75c5\u6bd2\u57f9\u517b\u7684\u5bf9\u6bd4"},"content":{"rendered":"\n

Original Investigation <\/p>\n\n\n\n

April 29, 2022<\/p>\n\n\n\n

Comparison of Home Antigen Testing With RT-PCR and Viral Culture During the Course of SARS-CoV-2 Infection<\/h1>\n\n\n\n

Victoria T.\u00a0Chu,\u00a0Noah G.\u00a0Schwartz,\u00a0Marisa A. P.\u00a0Donnelly,\u00a0et al,\u00a0for the COVID-19 Household Transmission Team<\/h3>\n\n\n\n

JAMA Intern Med.\u00a0<\/em>2022;182(7):701-709. <\/h3>\n\n\n\n

doi:10.1001\/jamainternmed.2022.1827<\/h3>\n\n\n\n

Key Points<\/p>\n\n\n\n

Question<\/strong>  How does the diagnostic performance of home antigen tests change during the course of SARS-CoV-2 infection?<\/p>\n\n\n\n

Findings<\/strong>  In this prospective cohort study of 225 adults and children with reverse transcription\u2013polymerase chain reaction (RT-PCR)\u2013confirmed SARS-CoV-2 infection, antigen test sensitivity was 64% and 84% when compared with same-day RT-PCR and viral culture, respectively. Antigen test sensitivity peaked 4 days after illness onset (77%); a second test 1 to 2 days later showed improved sensitivity (81%-85%).<\/p>\n\n\n\n

Meaning<\/strong>  The study results suggest that symptomatic individuals with an initial negative home antigen test result for SARS-CoV-2 infection should test again 1 to 2 days later because test sensitivity seems to peak several days after illness onset.<\/a><\/p>\n\n\n\n

Abstract<\/p>\n\n\n\n

Importance<\/strong>  As self-collected home antigen tests become widely available, a better understanding of their performance during the course of SARS-CoV-2 infection is needed.<\/p>\n\n\n\n

Objective<\/strong>  To evaluate the diagnostic performance of home antigen tests compared with reverse transcription\u2013polymerase chain reaction (RT-PCR) and viral culture by days from illness onset, as well as user acceptability.<\/p>\n\n\n\n

Design, Setting, and Participants<\/strong>  This prospective cohort study was conducted from January to May 2021 in San Diego County, California, and metropolitan Denver, Colorado. The convenience sample included adults and children with RT-PCR\u2013confirmed infection who used self-collected home antigen tests for 15 days and underwent at least 1 nasopharyngeal swab for RT-PCR, viral culture, and sequencing.<\/p>\n\n\n\n

Exposures<\/strong>  SARS-CoV-2 infection.<\/p>\n\n\n\n

Main Outcomes and Measures<\/strong>  The primary outcome was the daily sensitivity of home antigen tests to detect RT-PCR\u2013confirmed cases. Secondary outcomes included the daily percentage of antigen test, RT-PCR, and viral culture results that were positive, and antigen test sensitivity compared with same-day RT-PCR and cultures. Antigen test use errors and acceptability were assessed for a subset of participants.<\/p>\n\n\n\n

Results<\/strong>\u00a0\u00a0This study enrolled 225 persons with RT-PCR\u2013confirmed infection (median [range] age, 29 [1-83] years; 117 female participants [52%]; 10 [4%] Asian, 6 [3%] Black or African American, 50 [22%] Hispanic or Latino, 3 [1%] Native Hawaiian or Other Pacific Islander, 145 [64%] White, and 11 [5%] multiracial individuals) who completed 3044 antigen tests and 642 nasopharyngeal swabs. Antigen test sensitivity was 50% (95% CI, 45%-55%) during the infectious period, 64% (95% CI, 56%-70%) compared with same-day RT-PCR, and 84% (95% CI, 75%-90%) compared with same-day cultures. Antigen test sensitivity peaked 4 days after illness onset at 77% (95% CI, 69%-83%). Antigen test sensitivity improved with a second antigen test 1 to 2 days later, particularly early in the infection. Six days after illness onset, antigen test result positivity was 61% (95% CI, 53%-68%). Almost all (216 [96%]) surveyed individuals reported that they would be more likely to get tested for SARS-CoV-2 infection if home antigen tests were available over the counter.<\/p>\n\n\n\n

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Conclusions and Relevance<\/strong>  The results of this cohort study of home antigen tests suggest that sensitivity for SARS-CoV-2 was moderate compared with RT-PCR and high compared with viral culture. The results also suggest that symptomatic individuals with an initial negative home antigen test result for SARS-CoV-2 infection should test again 1 to 2 days later because test sensitivity peaked several days after illness onset and improved with repeated testing.<\/p>\n","protected":false},"excerpt":{"rendered":"

Original Investigation  April 29, 2022 Comparison […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[32,23],"tags":[],"_links":{"self":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22190"}],"collection":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=22190"}],"version-history":[{"count":1,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22190\/revisions"}],"predecessor-version":[{"id":22191,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=\/wp\/v2\/posts\/22190\/revisions\/22191"}],"wp:attachment":[{"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=22190"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=22190"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csccm.org.cn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=22190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}